rictor cko mice have decrease oligodendrocyte process branching at p8

PTEN in Autism and Neurodevelopmental Disorders

cKO mouse models have emerged as valuable tools to understand specific spatiotemporal roles of PTEN during development and its impact on human developmental disorders including ASD. Using these models, significant progress has been made in determining cell-autonomous and cell-intrinsic effects that result as a consequence of Pten deletion.

Wnt-Dependent Oligodendroglial-Endothelial Interactions ...

2020-12-23 · Introduction. Oligodendrocytes (OLs) are the myelinating cells of the central nervous system. During the process of myelination, developing oligodendrocyte precursors (OPCs) undergo dramatic changes in morphology and size as they differentiate, in some cases, achieving a 7,000-fold increase in membrane volume, to supply hundreds of myelin segments for nerve …

Wnt-Dependent Oligodendroglial-Endothelial Interactions ...

2020-10-20 · Chavali et al. demonstrate that oligodendroglial precursor density and their interactions with endothelial tip cells regulate white matter vascular development. In hypoxic brain injury, OPCs activate canonical Wnt signaling in angiogenic endothelial cells. Ablation of OPC-derived Wnt7 ligands results in disrupted white matter tip-cell angiogenesis and myelination …

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NG2 + OPCs in Sox2 cKO have no Sox2 expression ( B3 ) but distribute within the demyelination lesions in a similar pattern to that in Sox2 WT mice ( B2 ). C1–C3, Confocal images of NG2 and Sox10 double immunostaining ( C1, C2 ) and quantification of Sox10 + NG2 + OPCs ( C3 ).

Retinoic Acid Is Required for Oligodendrocyte Precursor ...

2020-1-1 · Myelination of the CNS relies on the production and differentiation of oligodendrocyte (OL) precursor cells (OPCs) into mature OLs. During the first month of postnatal life, OPCs that populate the corpus callosum (CC) arise from neural stem cells (NSCs) in the subcallosal subventricular zone (SVZ), and then differentiate to generate myelinating OLs. However, the …

Dystroglycan promotes filopodial formation and process ...

Oligodendrocytes that lack expression of the Lm-211 ligand, dystroglycan, also have deficits in oligodendrocyte branching (Eyermann et al., 2012), suggesting …

Deletion of Rictor in neural progenitor cells reveals ...

2013-1-1 · Rictor CKO mice are viable and have impaired mTORC2 signaling. To assess the contribution of Rictor in dorsal neural progenitor cells of the cortex, floxed Rictor mice were bred with Emx1 Cre animals to generate mice that are homozygous for the Rictor Flox-floxed allele and heterozygous for Emx1 Cre (Rictor CKO). Rictor CKO mice were born at expected Mendelian …

Murine models of IDH-wild-type glioblastoma exhibit ...

2020-7-22 · Importantly, Rictor deletion prolonged nearly 50% of the lifespan of p53 ∆E5–6 Rictor ∆/∆ CKO mice with malignant gliomas and GBMs, compared to p53 ∆E5–6 CKO mice. Importantly, Rictor ...

Deletion of Rictor in neural progenitor cells reveals ...

 · Rictor CKO mice have decreased mTORC2 signaling with decreased brain and body weight. (A) Representative immunoblots of control (con) versus Rictor CKO dorsal cortex extracts.(B) Significant decreases in Rictor, phosphorylated Akt at both serine 473 and threonine 308 residues and the indirect mTORC2 target pNDRG1 from dorsal brain extracts of Rictor …

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Finally, using vascular casting and micro-CT, we found that the TTVS is the dominant venous drainage in the early postnatal period (P8). Herein, we demonstrated that the TTVS is conserved between mice and humans, and we present a longitudinal study of its development.

Deletion of Rictor in neural progenitor cells reveals ...

2013-1-1 · Rictor CKO mice have small brains and bodies, normal lifespan and are fertile. Cortical layering is normal, but neurons are smaller than those in control brains. Seizures were not observed, although excessive slow activity was seen on electroencephalography. Rictor CKO mice are hyperactive and have reduced anxiety-like behavior.

LRP1 regulates peroxisome biogenesis and cholesterol ...

2017-12-18 · LRP1 protein levels in the brains of P10, P21, and P56 Lrp1 control and cKO OL mice were analyzed by Western blot analysis and revealed a partial loss of LRP1β (Figure 2—figure supplement 1b). The partial loss of LRP1β in …

YAP/TAZ-CDC42 signaling regulates vascular tip cell ...

2017-10-10 · New blood vessel formation is a physiological process seen in development, as well as in wound healing and tumorigenesis. Although the process of blood vasculature formation has been well documented, little is known about …

LKB1 Regulates Cerebellar Development by Controlling …

LKB1 conditional knockout mice (LKB1 Atoh1 CKO mice) were obtained and genotyped by PCR analysis (Fig. 1A). The amount of LKB1 protein was measured in the whole cerebellum using Western blots, and the results showed an obvious decrease of LKB1 protein in the LKB1 Atoh1 CKO mice compared with the wild-type mice ( Fig. 1B ).

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Deletion of Rictor in neural progenitor cells reveals ...

2012-10-9 · Rictor CKO mice are viable and have impaired mTORC2 signaling. To assess the contribution of Rictor in dorsal neural progenitor cells of the cortex, floxed Rictor mice were bred with Emx1 Cre animals to generate mice that are homozygous for the Rictor Flox-floxed allele and heterozygous for Emx1 Cre (Rictor CKO). Rictor CKO mice were born at expected Mendelian …

Rac1 Modulates Excitatory Synaptic Transmission in Mouse ...

2019-3-19 · Rac1 Modulates Synaptic Transmission in RGCs. Genotype screening by PCR showed a single 242-bp band in Rac1-cKO mice, while wild-type Rac1 +/+ mice showed a 115-bp band (Fig. S1A). The results of genotype screening of Chat-cre +/– mice are shown in Fig. S1B. The Rac1 protein expression levels in whole retinal extracts from Rac1-cKO mice were …

NMDA receptors control development of somatosensory ...

2021-3-4 · We have performed western blot of S1 at P8 and found GluN2A is expressed at P8. We have added "To address previous studies that found that GluN2A was expressed at lower levels during development we checked the expression of GluN2A in S1 at P8, and found that it was dramatically diminished in Emx1 cre/+; Grin2A fl/fl mice (Figure 6—figure ...

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Possible cause of HPPD: Hyperactive mTORC1

2021-4-22 · As a key regulator of autophagy, the mTOR plays an important role in autophagy, translation, cell growth and survival (Hwang et al., 2017).Mammalian target of rapamycin and autophagy are tightly bound within cells, and defects of mTOR and autophagy process might lead to a variety of human diseases (Hoeffer and Klann, 2010).Studies have shown that mTOR is …

LKB1 Regulates Cerebellar Development by Controlling …

2015-11-9 · The result showed that the total amount of Tbr2 protein was significantly increased in the P3.5 and P8 LKB1 Atoh1 CKO mice compared to the wild-type mice (Fig. 7C,C''). These findings suggested ...

Intracellular Signaling Pathway Regulation of Myelination ...

2021-12-31 · Canonical Wnt Signaling Pathway. The wingless and integration site (Wnt) intracellular signaling cascade is highly conserved throughout evolution and plays an integral role in animal development, growth, metabolism, and maintenance of stem cells (van Amerongen and Nusse, 2009) mammals, the canonical Wnt pathway consists of the extracellular Wnt …

10.1016/j.expneurol.2016.03.008 | 10.1016/j ...

2020-6-11 · BACE-1 is J.M. Gaesser, S.L. Fyffe-Maricich / Experimental Neurology 283 (2016) 501–511 505 an enzyme that cleaves neuregulin-1 type III (NRG1-III) and is an impor- CreRT2 mice to delete Raptor, Rictor, or Raptor/Rictor (Lebrun-Julien tant upstream enzyme that activates AKT signaling (Hu et al., 2006).

Mechanistic Target of Rapamycin Regulates the ...

2020-4-8 · We next examined MBP intensity in white matter in mTOR cKO and control mice at P7. As expected based on the reduction in CC1+ cells at this age in VWM, there was a significant reduction in MBP intensity (Fig. 8H–J; p = 0.030, t = 2.97, df = 5) and a decrease in white matter area in mTOR cKO mice (p = 0.013, t = 3.76, df = 5; Fig. 8K).

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Finally, using vascular casting and micro-CT, we found that the TTVS is the dominant venous drainage in the early postnatal period (P8). Herein, we demonstrated that the TTVS is conserved between mice and humans, and we present a longitudinal study of its development.

Translational Control of Myelin Basic Protein Expression ...

2015-5-20 · ERK2 CKO mice have a selective deficit in MBP expression early in the myelin repair process. Blocking the expression of pERK1/2 in cell culture hinders the ability of OPCs to transition to mature OLs (Fyffe-Maricich et al., …

Deletion of Rictor in neural progenitor cells reveals ...

Rictor CKO mice have decreased mTORC2 signaling with decreased brain and body weight. (A) Representative immunoblots of control (con) versus Rictor CKO dorsal cortex extracts.(B) Significant decreases in Rictor, phosphorylated Akt at both serine 473 and threonine 308 residues and the indirect mTORC2 target pNDRG1 from dorsal brain extracts of Rictor CKO mice when …